All the seminars, past and upcoming
Start Monday October 10th (new date) at 4pm (CET, Berlin/Paris)
(3 pm London/Lisbon, 10am New-York, 7am San Francisco, 5pm Tel Aviv)
Link to the Zoom conference: https://univ-grenoble-alpes-fr.zoom.us/j/99594257935?pwd=ZmIyTFgyVWw0eFpmbFdmMG1yQkFsZz09
Link to the YouTube stream: https://youtu.be/TdJFitndjk0
Main Speaker: Marc Friedländer,
Associate professor, Principal investigator in quantitative RNA biology, Stockholm, Sweden (Link)
agoTRIBE experimentally maps microRNA targets transcriptome-wide in single cells
MicroRNAs are gene regulatory molecules that play important roles in numerous biological processes including human health. The function of a given microRNA is defined by its selection of target transcripts, yet current state-of-the-art experimental methods to identify microRNA targets are laborious and require millions of cells. We have overcome these limitations by fusing the microRNA effector protein Argonaute2 to the RNA editing domain of ADAR2, allowing for the first time the detection of microRNA targets transcriptome-wide in single cells. Our agoTRIBE method reports functional microRNA targets which are additionally supported by evolutionary sequence conservation. As a proof-of-principle, we study microRNA interactions in single cells, and find substantial differential targeting across the cell cycle. Lastly, agoTRIBE additionally provides transcriptome-wide measurements of RNA abundance and will allow the deconvolution of microRNA targeting in complex tissues at the single-cell level.
Short session speaker (8 minutes long):
Matthias Zytnicki, senior researcher, Toulouse, France (Link)
Title: 4 tools for small RNA-seq (mapping, quantification, differential expression)
CEA is a French government-funded technological research organisation in four main areas: low-carbon energies, defense and security, information technologies and health technologies. A prominent player in the European Research Area, it is involved in setting up collaborative projects with many partners around the world.